Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity

Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G
Viruses 14(9):1974 (2022 Sep 06)

doi: 10.3390/v14091974

Submitted to SASBDB: 2021 Oct 19 Published in SASBDB: 2022 Sep 10

SASDMX6 – 40-mer single stranded DNA inhibitor of DNA dC->dU-editing enzyme APOBEC3G Sample: 40-mer single stranded inhibitory DNA monomer, 12 kDa DNA Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 8 RgGuinier 3.2 nm Dmax 11.8 nm VolumePorod 26 nm3

SASDMY6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length Sample: DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6 RgGuinier 4.2 nm Dmax 13.3 nm VolumePorod 350 nm3

SASDMZ6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: tetramer Sample: DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein 40-mer single stranded inhibitory DNA dimer, 24 kDa DNA Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6 RgGuinier 4.7 nm Dmax 16.2 nm VolumePorod 395 nm3

SASDM27 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: monomer Sample: 40-mer single stranded inhibitory DNA monomer, 12 kDa DNA DNA dC->dU-editing enzyme APOBEC-3G monomer, 46 kDa Homo sapiens protein Buffer: 50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6 RgGuinier 3.1 nm Dmax 10.0 nm VolumePorod 118 nm3