Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
npj Biofilms and Microbiomes 8(1) (2022 Feb 25)

doi: 10.1038/s41522-022-00272-5

Submitted to SASBDB: 2021 Oct 9 Published in SASBDB: 2022 Mar 1

SASDLW2 – Accessory colonization factor SslE at pH 4.4 Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 4.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27 RgGuinier 3.9 nm Dmax 13.7 nm VolumePorod 248 nm3

SASDLV2 – Accessory colonization factor SslE at pH 7.4 Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27 RgGuinier 4.0 nm Dmax 14.1 nm VolumePorod 244 nm3

SASDMU6 – The N1 domain of accessory colonization factor SslE Sample: Accessory colonization factor monomer, 17 kDa Escherichia coli (strain … protein Buffer: 20 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21 RgGuinier 2.0 nm Dmax 7.0 nm VolumePorod 28 nm3

SASDMV6 – The N2 domain of accessory colonization factor SslE Sample: Accessory colonization factor monomer, 23 kDa Escherichia coli (strain … protein Buffer: 20 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21 RgGuinier 2.2 nm Dmax 7.7 nm VolumePorod 42 nm3

SASDMW6 – The N3-M60 domain of accessory colonization factor SslE Sample: Accessory colonization factor monomer, 121 kDa Escherichia coli (strain … protein Buffer: 20 mM Tris, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21 RgGuinier 3.4 nm Dmax 10.5 nm VolumePorod 171 nm3