Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome

Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N
Journal of Biological Chemistry :101896 (2022 Apr)

doi: 10.1016/j.jbc.2022.101896
Submitted to SASBDB: 2021 Dec 6
Published in SASBDB:

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
RgGuinier 5.4 nm
Dmax 20.3 nm
VolumePorod 93 nm3

SASDN22 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 45 nm3

SASDN32 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 41 nm3

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 84 nm3

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
RgGuinier 2.1 nm
Dmax 10.3 nm
VolumePorod 42 nm3

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 35 nm3