Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate

Adriana Erica Miele

Submitted to SASBDB: 2022 Jan 14 Published in SASBDB: 2026 Jan 14

SASDN24 – Heparin oligosaccharide DP10 Sample: Heparine oligosaccharide of 10 sugar units (5 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 3 kDa Sus scrofa domesticus Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 May 6 RgGuinier 1.3 nm Dmax 5.7 nm

SASDN34 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, pH 7.4 Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26 RgGuinier 1.5 nm Dmax 8.5 nm

SASDN44 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26 RgGuinier 1.5 nm Dmax 6.8 nm

SASDN54 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26 RgGuinier 1.4 nm Dmax 8.6 nm

SASDN64 – Heparin oligosaccharide DP12 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 12 sugar units (6 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 4 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 26 RgGuinier 1.5 nm Dmax 6.8 nm

SASDN74 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, pH 7.4 Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.8 nm Dmax 6.3 nm

SASDN84 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.8 nm Dmax 7.7 nm

SASDN94 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.8 nm Dmax 7.9 nm

SASDNA4 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4 Sample: Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan monomer, 5 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.8 nm Dmax 7.5 nm

SASDNB4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 4.8 nm Dmax 29.7 nm

SASDNC4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 4.8 nm Dmax 23.9 nm

SASDND4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 5mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.7 nm Dmax 23.7 nm

SASDNE4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 10 mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 2.4 nm Dmax 20.8 nm

SASDNF4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 20 mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.4 nm Dmax 22.2 nm

SASDNG4 – Heparin oligosaccharide in 50 mM Tris/HCl pH 7.4, 40 mg/mL Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 1.0 nm Dmax 22.1 nm

SASDNH4 – Heparin oligosaccharide in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4 Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 4.6 nm Dmax 23.9 nm

SASDNJ4 – Heparin oligosaccharide in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4 Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 5.1 nm Dmax 27.5 nm

SASDNK4 – Heparin oligosaccharide in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4 Sample: Heparin monomer, 14 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 5.1 nm Dmax 30.8 nm

SASDNL4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 1.25 mg/mL Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.2 nm Dmax 29.6 nm

SASDNM4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.2 nm Dmax 26.2 nm

SASDNN4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 5 mg/mL Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.1 nm Dmax 19.8 nm

SASDNP4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4 Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.9 nm Dmax 22.2 nm

SASDNQ4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4 Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.8 nm Dmax 22.7 nm

SASDNR4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4 Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13 RgGuinier 3.8 nm Dmax 20.6 nm