Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS).

Schamber T, Binas O, Schlundt A, Wacker A, Schwalbe H
Chembiochem (2021 Nov 30)

PMID: 34847270 doi: 10.1002/cbic.202100564

Submitted to SASBDB: 2021 Dec 6 Published in SASBDB: 2021 Dec 7

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 1.5 nm Dmax 4.8 nm VolumePorod 14 nm3

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 1.8 nm Dmax 5.8 nm VolumePorod 15 nm3

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 2.2 nm Dmax 8.8 nm VolumePorod 19 nm3

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 4.1 nm Dmax 16.9 nm VolumePorod 56 nm3

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 4.2 nm Dmax 18.1 nm VolumePorod 57 nm3

SASDMW9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ and Gdn ligand Sample: Guanidin-II riboswitch 49mer hexamer, 95 kDa Escherichia coli RNA Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2 Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13 RgGuinier 8.8 nm Dmax 33.6 nm VolumePorod 442 nm3