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39 hits found for Schlundt

SASDVX2 – Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

full stem-loop 5 of SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: full stem-loop 5 of SARS-CoV-2 5'genomic end monomer, 48 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 4.3 nm
Dmax 13.8 nm

SASDVY2 – Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5a from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5a from SARS-CoV-2 5'genomic end monomer, 11 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 1.9 nm
Dmax 6.0 nm

SASDVC3 – Sub-element stem-loop 5b within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5b from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5b from SARS-CoV-2 5'genomic end monomer, 8 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 3
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 1.4 nm
Dmax 4.3 nm

SASDNW5 – apo Roquin-1 (coreROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 experimental SAS data
Roquin-1 Kratky plot
Sample: Roquin-1 monomer, 18 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 35 nm3

SASDNX5 – apo Roquin-1 (extROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 extROQ experimental SAS data
Roquin-1 extROQ Kratky plot
Sample: Roquin-1 extROQ monomer, 35 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.8 nm
Dmax 14.0 nm
VolumePorod 53 nm3

SASDNY5 – apo Roquin-1 (Nterm ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm experimental SAS data
Roquin-1 Nterm Kratky plot
Sample: Roquin-1 Nterm monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.4 nm
Dmax 15.0 nm
VolumePorod 88 nm3

SASDNZ5 – apo Roquin-1 (Nterm A-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm Amut (K220A, K239A, R260A) experimental SAS data
Roquin-1 Nterm Amut (K220A, K239A, R260A) Kratky plot
Sample: Roquin-1 Nterm Amut (K220A, K239A, R260A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 80 nm3

SASDN26 – apo Roquin-1 (Nterm B-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) experimental SAS data
Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) Kratky plot
Sample: Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.5 nm
Dmax 15.0 nm
VolumePorod 92 nm3

SASDN36 – Ox40 RNA (untranslated region, UTR) full-length

Ox40 3'UTR full-length experimental SAS data
Ox40 3'UTR full-length Kratky plot
Sample: Ox40 3'UTR full-length monomer, 50 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 115 nm3

SASDN46 – Ox40 RNA (untranslated region, UTR) alternative decay element (ADE)

Ox40 3 UTR ADE experimental SAS data
Ox40 3 UTR ADE Kratky plot
Sample: Ox40 3 UTR ADE monomer, 16 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 21 nm3

SASDN56 – Ox40 RNA (untranslated region, UTR) constitutive decay element (CDE)

Ox40 3 UTR CDE experimental SAS data
Ox40 3 UTR CDE Kratky plot
Sample: Ox40 3 UTR CDE monomer, 9 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.0 nm
Dmax 9.5 nm
VolumePorod 16 nm3

SASDN66 – Ox40 RNA (untranslated region, UTR) alternative and constitutive decay element (ADE-CDE)

Ox40 3 UTR ADE-CDE experimental SAS data
Ox40 3 UTR ADE-CDE Kratky plot
Sample: Ox40 3 UTR ADE-CDE monomer, 24 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 26 nm3

SASDN76 – Ox40 RNA (untranslated region, UTR) Bulge

Ox40 3 UTR Bulge experimental SAS data
Ox40 3 UTR Bulge Kratky plot
Sample: Ox40 3 UTR Bulge monomer, 11 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.8 nm
Dmax 6.5 nm
VolumePorod 17 nm3

SASDN86 – Ox40 RNA (untranslated region, UTR) Bulge with alternative decay element (Bulge-ADE)

Ox40 3 UTR Bulge-ADE experimental SAS data
Ox40 3 UTR Bulge-ADE Kratky plot
Sample: Ox40 3 UTR Bulge-ADE monomer, 27 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 37 nm3

SASDN96 – Ox40 RNA (untranslated region, UTR) Bulge with alternative and constitutive decay elements (Bulge-ADE-CDE)

Ox40 3 UTR Bulge-ADE-CDE experimental SAS data
Ox40 3 UTR Bulge-ADE-CDE Kratky plot
Sample: Ox40 3 UTR Bulge-ADE-CDE monomer, 35 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 46 nm3

SASDNA6 – Ox40 RNA (untranslated region, UTR) constitutive decay element (CDEshort)

Ox40 3 UTR CDEshort experimental SAS data
Ox40 3 UTR CDEshort Kratky plot
Sample: Ox40 3 UTR CDEshort monomer, 5 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.5 nm

SASDNB6 – Ox40 RNA (untranslated region, UTR) terminal stem (Terminusuucg)

Ox40 3 UTR Terminusuucg experimental SAS data
Ox40 3 UTR Terminusuucg Kratky plot
Sample: Ox40 3 UTR Terminusuucg monomer, 10 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 16 nm3

SASDNC6 – Ox40 RNA (untranslated region, UTR), alternative decay element (ADEshort)

Ox40 3 UTR ADEshort experimental SAS data
Ox40 3 UTR ADEshort Kratky plot
Sample: Ox40 3 UTR ADEshort monomer, 7 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.7 nm
Dmax 9.0 nm
VolumePorod 12 nm3

SASDRV7 – SARS-CoV-2 5' untranslated region stem-loop 4 RNA (5_SL4) in phosphate buffer

stem-loop 4 from SARS-CoV-2 5'-UTR experimental SAS data
stem-loop 4 from SARS-CoV-2 5'-UTR Kratky plot
Sample: stem-loop 4 from SARS-CoV-2 5'-UTR monomer, 14 kDa SARS coronavirus 2-Wuhan RNA
Buffer: 25 mM potassium phosphate pH 6.5, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
High-resolution structure of stem-loop 4 from the 5'-UTR of SARS-CoV-2 solved by solution state NMR. Nucleic Acids Res (2023)
Vögele J, Hymon D, Martins J, Ferner J, Jonker HRA, Hargrove AE, Weigand JE, Wacker A, Schwalbe H, Wöhnert J, Duchardt-Ferner E
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 14 nm3

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 15 nm3

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 2.2 nm
Dmax 8.8 nm
VolumePorod 19 nm3

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 4.1 nm
Dmax 16.9 nm
VolumePorod 56 nm3

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 57 nm3

SASDMW9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer hexamer, 95 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 8.8 nm
Dmax 33.6 nm
VolumePorod 442 nm3

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.6 nm
Dmax 6.1 nm
VolumePorod 23 nm3

SASDPL6 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDPM6 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.0 nm
Dmax 7.1 nm
VolumePorod 22 nm3

SASDPN6 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.8 nm
Dmax 10.2 nm
VolumePorod 33 nm3

SASDPP6 – 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.5 nm
Dmax 5.4 nm
VolumePorod 13 nm3

SASDPQ6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 38 nm3

SASDPR6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 51 nm3

SASDPS6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinAU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 32 nm3

SASDPT6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions (mixture)

NucleoproteinStem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 25 nm3

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 26 nm3

SASDR43 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in HEPES conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 28 nm3

SASDR53 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in HEPES conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.7 nm
VolumePorod 22 nm3

SASDR63 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in HEPES conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 3.1 nm
Dmax 11.7 nm
VolumePorod 35 nm3

SASDR73 – 5'-genomic RNA AU extension of SARS-CoV-2 in HEPES conditions

AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.7 nm
Dmax 7.0 nm
VolumePorod 13 nm3