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42 hits found for Schlundt

SASDVX2 – Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

full stem-loop 5 of SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: full stem-loop 5 of SARS-CoV-2 5'genomic end monomer, 48 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 4.3 nm
Dmax 13.8 nm

SASDVY2 – Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5a from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5a from SARS-CoV-2 5'genomic end monomer, 11 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 1.9 nm
Dmax 6.0 nm

SASDVC3 – Sub-element stem-loop 5b within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5b from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5b from SARS-CoV-2 5'genomic end monomer, 8 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 3
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Schlundt A, Schwalbe H
RgGuinier 1.4 nm
Dmax 4.3 nm

SASDVZ3 – SARS-CoV2 RNA pseudoknot at 4 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Schlundt A, Weigand J, Göbel M, Wacker A, Schwalbe H
RgGuinier 2.3 nm
Dmax 7.9 nm
VolumePorod 29 nm3

SASDV24 – SARS-CoV2 RNA pseudoknot at 2 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Schlundt A, Weigand J, Göbel M, Wacker A, Schwalbe H
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 32 nm3

SASDV34 – SARS-CoV2 RNA pseudoknot at 1 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Schlundt A, Weigand J, Göbel M, Wacker A, Schwalbe H
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 32 nm3

SASDNW5 – apo Roquin-1 (coreROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 experimental SAS data
Roquin-1 Kratky plot
Sample: Roquin-1 monomer, 18 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 35 nm3

SASDNX5 – apo Roquin-1 (extROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 extROQ experimental SAS data
Roquin-1 extROQ Kratky plot
Sample: Roquin-1 extROQ monomer, 35 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.8 nm
Dmax 14.0 nm
VolumePorod 53 nm3

SASDNY5 – apo Roquin-1 (Nterm ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm experimental SAS data
Roquin-1 Nterm Kratky plot
Sample: Roquin-1 Nterm monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.4 nm
Dmax 15.0 nm
VolumePorod 88 nm3

SASDNZ5 – apo Roquin-1 (Nterm A-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm Amut (K220A, K239A, R260A) experimental SAS data
Roquin-1 Nterm Amut (K220A, K239A, R260A) Kratky plot
Sample: Roquin-1 Nterm Amut (K220A, K239A, R260A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 80 nm3

SASDN26 – apo Roquin-1 (Nterm B-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) experimental SAS data
Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) Kratky plot
Sample: Roquin-1 Nterm Bmut (R135E, K136E, D322A, K323A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.5 nm
Dmax 15.0 nm
VolumePorod 92 nm3

SASDN36 – Ox40 RNA (untranslated region, UTR) full-length

Ox40 3'UTR full-length experimental SAS data
Ox40 3'UTR full-length Kratky plot
Sample: Ox40 3'UTR full-length monomer, 50 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 115 nm3

SASDN46 – Ox40 RNA (untranslated region, UTR) alternative decay element (ADE)

Ox40 3 UTR ADE experimental SAS data
Ox40 3 UTR ADE Kratky plot
Sample: Ox40 3 UTR ADE monomer, 16 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.2 nm
VolumePorod 21 nm3

SASDN56 – Ox40 RNA (untranslated region, UTR) constitutive decay element (CDE)

Ox40 3 UTR CDE experimental SAS data
Ox40 3 UTR CDE Kratky plot
Sample: Ox40 3 UTR CDE monomer, 9 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.0 nm
Dmax 9.5 nm
VolumePorod 16 nm3

SASDN66 – Ox40 RNA (untranslated region, UTR) alternative and constitutive decay element (ADE-CDE)

Ox40 3 UTR ADE-CDE experimental SAS data
Ox40 3 UTR ADE-CDE Kratky plot
Sample: Ox40 3 UTR ADE-CDE monomer, 24 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 26 nm3

SASDN76 – Ox40 RNA (untranslated region, UTR) Bulge

Ox40 3 UTR Bulge experimental SAS data
Ox40 3 UTR Bulge Kratky plot
Sample: Ox40 3 UTR Bulge monomer, 11 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.8 nm
Dmax 6.5 nm
VolumePorod 17 nm3

SASDN86 – Ox40 RNA (untranslated region, UTR) Bulge with alternative decay element (Bulge-ADE)

Ox40 3 UTR Bulge-ADE experimental SAS data
Ox40 3 UTR Bulge-ADE Kratky plot
Sample: Ox40 3 UTR Bulge-ADE monomer, 27 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 37 nm3

SASDN96 – Ox40 RNA (untranslated region, UTR) Bulge with alternative and constitutive decay elements (Bulge-ADE-CDE)

Ox40 3 UTR Bulge-ADE-CDE experimental SAS data
Ox40 3 UTR Bulge-ADE-CDE Kratky plot
Sample: Ox40 3 UTR Bulge-ADE-CDE monomer, 35 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 3.4 nm
Dmax 11.0 nm
VolumePorod 46 nm3

SASDNA6 – Ox40 RNA (untranslated region, UTR) constitutive decay element (CDEshort)

Ox40 3 UTR CDEshort experimental SAS data
Ox40 3 UTR CDEshort Kratky plot
Sample: Ox40 3 UTR CDEshort monomer, 5 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.5 nm

SASDNB6 – Ox40 RNA (untranslated region, UTR) terminal stem (Terminusuucg)

Ox40 3 UTR Terminusuucg experimental SAS data
Ox40 3 UTR Terminusuucg Kratky plot
Sample: Ox40 3 UTR Terminusuucg monomer, 10 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 16 nm3

SASDNC6 – Ox40 RNA (untranslated region, UTR), alternative decay element (ADEshort)

Ox40 3 UTR ADEshort experimental SAS data
Ox40 3 UTR ADEshort Kratky plot
Sample: Ox40 3 UTR ADEshort monomer, 7 kDa Mus musculus RNA
Buffer: 50 mM KCl, 25 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
...Schlundt A
RgGuinier 1.7 nm
Dmax 9.0 nm
VolumePorod 12 nm3

SASDRV7 – SARS-CoV-2 5' untranslated region stem-loop 4 RNA (5_SL4) in phosphate buffer

stem-loop 4 from SARS-CoV-2 5'-UTR experimental SAS data
stem-loop 4 from SARS-CoV-2 5'-UTR Kratky plot
Sample: stem-loop 4 from SARS-CoV-2 5'-UTR monomer, 14 kDa SARS coronavirus 2-Wuhan RNA
Buffer: 25 mM potassium phosphate pH 6.5, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
High-resolution structure of stem-loop 4 from the 5'-UTR of SARS-CoV-2 solved by solution state NMR. Nucleic Acids Res (2023)
Vögele J, Hymon D, Martins J, Ferner J, Jonker HRA, Hargrove AE, Weigand JE, Wacker A, Schwalbe H, Wöhnert J, Duchardt-Ferner E
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 14 nm3

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 15 nm3

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 2.2 nm
Dmax 8.8 nm
VolumePorod 19 nm3

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 4.1 nm
Dmax 16.9 nm
VolumePorod 56 nm3

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 57 nm3

SASDMW9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer hexamer, 95 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Schlundt A, Wacker A, Schwalbe H
RgGuinier 8.8 nm
Dmax 33.6 nm
VolumePorod 442 nm3

SASDPK6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in phosphate conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.6 nm
Dmax 6.1 nm
VolumePorod 23 nm3

SASDPL6 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 24 nm3

SASDPM6 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.0 nm
Dmax 7.1 nm
VolumePorod 22 nm3

SASDPN6 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.8 nm
Dmax 10.2 nm
VolumePorod 33 nm3

SASDPP6 – 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
RNAMASONRY model
Sample: AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.5 nm
Dmax 5.4 nm
VolumePorod 13 nm3

SASDPQ6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 38 nm3

SASDPR6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 51 nm3

SASDPS6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA AU extension of SARS-CoV-2 in phosphate conditions

NucleoproteinAU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 16
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 32 nm3

SASDPT6 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in phosphate conditions (mixture)

NucleoproteinStem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 25 nm3

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 26 nm3

SASDR43 – 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in HEPES conditions

Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 28 nm3

SASDR53 – 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in HEPES conditions

Stem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 2.1 nm
Dmax 7.7 nm
VolumePorod 22 nm3

SASDR63 – 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in HEPES conditions

Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 3.1 nm
Dmax 11.7 nm
VolumePorod 35 nm3

SASDR73 – 5'-genomic RNA AU extension of SARS-CoV-2 in HEPES conditions

AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
...Schlundt A
RgGuinier 1.7 nm
Dmax 7.0 nm
VolumePorod 13 nm3