Production and characterisation of modularly deuterated UBE2D1–Ub conjugate by small angle neutron and X-ray scattering

Pietras Z, Duff A, Morad V, Wood K, Jeffries C, Sunnerhagen M
European Biophysics Journal (2022 Oct 26)

doi: 10.1007/s00249-022-01620-1 		Submitted to SASBDB: 2022 Jun 6
Published in SASBDB:

SASDP34 – Ubiquitin-conjugating enzyme E2 conjugated to deuterated Ubiquitin (SANS data at 0% v/v D2O, plus additional SANS with contrast variation and SAXS datasets)

Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S)Ubiquitin experimental SAS data
MONSA model
Sample: Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S) monomer, 17 kDa Homo sapiens protein
Ubiquitin monomer, 9 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2019 May 24
RgGuinier 2.1 nm
Dmax 7.4 nm