RNF8 E3 Ubiquitin Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment.

Hodge CD, Ismail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
J Biol Chem 291(18):9396-410 (2016 Apr 29)

PMID: 26903517 doi: 10.1074/jbc.M116.715698

Submitted to SASBDB: 2016 Feb 11 Published in SASBDB: 2016 Aug 31

SASDBR3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin Sample: E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8 RgGuinier 4.5 nm Dmax 18.9 nm VolumePorod 119 nm3

SASDBS3 – Wild type RNF8 complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin Sample: E3 ubiquitin-protein ligase RNF8 dimer, 35 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8 RgGuinier 5.4 nm Dmax 23.8 nm VolumePorod 214 nm3

SASDBT3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin Sample: Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8 RgGuinier 4.3 nm Dmax 18.9 nm VolumePorod 111 nm3

SASDBU3 – RNF8 (L451D mutant) complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin Sample: Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa Homo sapiens protein Polyubiquitin-C dimer, 17 kDa Homo sapiens protein Ubiquitin-conjugating enzyme E2 variant 2 dimer, 34 kDa Homo sapiens protein E3 ubiquitin-protein ligase RNF8 mutant (L451D) dimer, 35 kDa Homo sapiens protein Buffer: 20 mM HEPES 200 mM NaCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8 RgGuinier 5.2 nm Dmax 23.8 nm VolumePorod 192 nm3