The N-terminus of obscurin is flexible in solution.

Mauriello GE, Moncure GE, Nowzari RA, Miller CJ, Wright NT
Proteins (2022 Oct 28)

PMID: 36306263 doi: 10.1002/prot.26442

Submitted to SASBDB: 2022 Mar 30 Published in SASBDB: 2022 Dec 20

SASDNV7 – Obscurin Ig domains 11/12 at neutral pH Sample: Obscurin monomer, 21 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 18 RgGuinier 3.7 nm Dmax 18.0 nm VolumePorod 66 nm3

SASDNU7 – Obscurin Ig domains 12/13 at neutral pH Sample: Obscurin monomer, 21 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 18 RgGuinier 2.8 nm Dmax 10.5 nm VolumePorod 28 nm3

SASDNW7 – Obscurin Ig domains 12/13, short at neutral pH Sample: Obscurin Ig domains 12/13 short monomer, 21 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 20 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 25 nm3

SASDNX7 – Obscurin Ig domains 12/13, long at neutral pH Sample: Obscurin Ig domains 12/13 long monomer, 22 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 20 RgGuinier 2.9 nm Dmax 10.0 nm VolumePorod 26 nm3