Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module.

Garcia-Rodriguez G, Girardin Y, Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
Sci Adv 10(1):eadj2403 (2024 Jan 5)

PMID: 38181072 doi: 10.1126/sciadv.adj2403

Submitted to SASBDB: 2022 May 17 Published in SASBDB: 2024 Jan 16

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18 RgGuinier 3.2 nm Dmax 10.0 nm VolumePorod 140 nm3

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4 RgGuinier 3.3 nm Dmax 10.5 nm VolumePorod 160 nm3

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18 RgGuinier 3.2 nm Dmax 10.0 nm VolumePorod 150 nm3

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin, 25 kDa Vibrio cholerae serotype … protein Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 6 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 140 nm3