Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel.

Lycksell M, Rovšnik U, Hanke A, Martel A, Howard RJ, Lindahl E
Proc Natl Acad Sci U S A 119(50):e2210669119 (2022 Dec 13)
PMID: 36480474
doi: 10.1073/pnas.2210669119 		Submitted to SASBDB: 2022 Feb 7
Published in SASBDB:

SASDNG5 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel with calcium measured by paused-flow SEC-SANS

Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
GROMACS model
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
RgGuinier 5.2 nm
Dmax 16.8 nm
VolumePorod 530 nm3

SASDNH5 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel without calcium measured by paused-flow SEC-SANS

Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Kratky plot
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM EDTA, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 540 nm3