Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP.

Stiegler AL, Vish KJ, Boggon TJ
Structure (2022 Nov 16)

PMID: 36417908 doi: 10.1016/j.str.2022.10.009

Submitted to SASBDB: 2022 Jun 27 Published in SASBDB: 2022 Nov 26

SASDPM4 – Apo SH2-SH3-SH2 domains from p120RasGAP Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8 350 mM NaCl 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12 RgGuinier 2.6 nm Dmax 10.3 nm VolumePorod 41 nm3

SASDPN4 – Doubly Phosphorylated p190RhoGAP Peptide Bound to p120RasGAP's SH2-SH3-SH2 Domains Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8 350 mM NaCl 1 mM DTT, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 11 RgGuinier 2.4 nm Dmax 7.9 nm VolumePorod 41 nm3