Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation

Hsu J, Chen J, Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M
Frontiers in Molecular Biosciences 9 (2022 Apr 19)

doi: 10.3389/fmolb.2022.871499

Submitted to SASBDB: 2022 Mar 4 Published in SASBDB: 2022 Apr 20

SASDNF6 – full-length MERS CoV N -protein complexed with 5-propoxy-1H-indole (P4-1 compound) Sample: Nucleoprotein dodecamer, 548 kDa Middle East respiratory … protein 5-(Propoxy)-1H-indole dodecamer, 2 kDa Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 22 RgGuinier 6.4 nm Dmax 22.0 nm VolumePorod 896 nm3

SASDNG6 – full-length MERS CoV N-protein complexed with 5-Isopropoxy-1H-indole (P4-2 compound) Sample: Nucleoprotein dodecamer, 548 kDa Middle East respiratory … protein 5-Isopropoxy-1H-indole dodecamer, 2 kDa Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 22 RgGuinier 6.4 nm Dmax 22.2 nm VolumePorod 911 nm3

SASDNH6 – full-length MERS CoV N-protein complexed with 5-(2-fluoroethoxy)-1H-indole (P4-3 compound) Sample: Nucleoprotein tetramer, 183 kDa Middle East respiratory … protein 5-(2-fluoroethoxy)-1H-indole tetramer, 1 kDa Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 26 RgGuinier 5.7 nm Dmax 19.1 nm VolumePorod 477 nm3

SASDNJ6 – full-length MERS CoV N-protein complexed with 5-(2-methoxyethoxy)-1H-indole (P4-4 compound) Sample: Nucleoprotein tetramer, 183 kDa Middle East respiratory … protein 5-(2-methoxyethoxy)-1H-indole tetramer, 1 kDa Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5 Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 26 RgGuinier 6.0 nm Dmax 18.5 nm VolumePorod 500 nm3