Structure of SALL4 zinc finger domain reveals link between AT-rich DNA binding and Okihiro syndrome.

Watson JA, Pantier R, Jayachandran U, Chhatbar K, Alexander-Howden B, Kruusvee V, Prendecki M, Bird A, Cook AG
Life Sci Alliance 6(3) (2023 Mar)
PMID: 36635047
doi: 10.26508/lsa.202201588 		Submitted to SASBDB: 2022 Jun 12
Published in SASBDB:

SASDP64 – SALL4 Zinc Finger Cluster 4

Sal-like protein 4 experimental SAS data
DAMMIN model
Sample: Sal-like protein 4 monomer, 8 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 6
RgGuinier 2.1 nm
Dmax 5.8 nm
VolumePorod 11 nm3

SASDP74 – AT-rich DNA

AT-rich dsDNA experimental SAS data
DAMMIN model
Sample: AT-rich dsDNA monomer, 7 kDa DNA
Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 6
RgGuinier 1.7 nm
Dmax 4.6 nm
VolumePorod 12 nm3

SASDP84 – SALL4 Zinc Finger Cluster 4 bound to AT-rich DNA

Sal-like protein 4AT-rich dsDNA experimental SAS data
OTHER model
Sample: Sal-like protein 4 monomer, 8 kDa Mus musculus protein
AT-rich dsDNA monomer, 7 kDa DNA
Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 6
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 14 nm3