Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR

Bondarev N, Bagaeva D, Bazhenov S, Buben M, Bulushova N, Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I
Biochemical and Biophysical Research Communications :149319 (2023 Nov)

doi: 10.1016/j.bbrc.2023.149319

Submitted to SASBDB: 2022 Jul 7 Published in SASBDB: 2023 Nov 27

SASDPQ5 – His-tagged L-methionine gamma-lyase from Clostridium tetani Sample: L-methionine gamma-lyase tetramer, 181 kDa Clostridium tetani protein Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19 RgGuinier 4.0 nm Dmax 14.9 nm VolumePorod 232 nm3

SASDPS5 – L-methionine gamma-lyase from Clostridium sporogenes fused with S3 domain of the Vaccinia virus growth factor Sample: L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain tetramer, 183 kDa Clostridium sporogenes protein Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19 RgGuinier 5.2 nm Dmax 17.3 nm VolumePorod 303 nm3

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes Sample: L-methionine gamma-lyase (K272S) tetramer, 174 kDa Clostridium sporogenes protein Buffer: PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4 Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19 RgGuinier 3.7 nm Dmax 14.5 nm VolumePorod 211 nm3