Architecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation

Juyoux P, Galdadas I, Gobbo D, von Velsen J, Pelosse M, Tully M, Vadas O, Gervasio F, Pellegrini E, Bowler M
Science 381(6663):1217-1225 (2023 Sep 15)

doi: 10.1126/science.add7859

Submitted to SASBDB: 2023 Feb 22 Published in SASBDB: 2023 Mar 9

SASDRC6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with ADP-AlF4 Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein Adenosine 5'-Diphosphate Tetrafluoroaluminate monomer, 1 kDa Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15 RgGuinier 3.2 nm Dmax 12.1 nm VolumePorod 113 nm3

SASDRD6 – Dual specificity mitogen-activated protein kinase kinase 6 (MKK6GRA) bound to mitogen-activated protein kinase 14 (p38α) with AMP-PCP Sample: Mitogen-activated protein kinase 14 monomer, 40 kDa Homo sapiens protein Dual specificity mitogen-activated protein kinase kinase 6 monomer, 42 kDa synthetic construct protein 5′‐adenylyl methylenediphosphonate monomer, 1 kDa Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 5 % glycerol, 0.5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 15 RgGuinier 4.2 nm Dmax 14.0 nm VolumePorod 116 nm3