Structural insights into the substrate binding mechanism of the class I dehydratase MadB

Knospe C, Ortiz J, Reiners J, Kedrov A, Gertzen C, Smits S, Schmitt L
Communications Biology 8(1) (2025 Jul 09)

doi: 10.1038/s42003-025-08454-5 		Submitted to SASBDB: 2023 Feb 28
Published in SASBDB:

SASDRX6 – lantibiotic dehydratase MadB (apo)

Thiopeptide-type bacteriocin biosynthesis domain containing protein experimental SAS data
GASBOR model
Sample: Thiopeptide-type bacteriocin biosynthesis domain containing protein dimer, 250 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 1 % (v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
RgGuinier 4.5 nm
Dmax 13.6 nm
VolumePorod 440 nm3

SASDRY6 – lantibiotic dehydratase MadB bound to truncated maddinglicin with a full leader peptide (Leader3)

Thiopeptide-type bacteriocin biosynthesis domain containing proteinLantibiotic, gallidermin/nisin family experimental SAS data
GASBOR model
Sample: Thiopeptide-type bacteriocin biosynthesis domain containing protein dimer, 250 kDa Clostridium sp. Maddingley … protein
Lantibiotic, gallidermin/nisin family monomer, 3 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 1 % (v/v) Glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 3
RgGuinier 4.5 nm
Dmax 13.8 nm
VolumePorod 445 nm3