Structure of the lantibiotic dehydratase MadB

Jens Reiners Submitted to SASBDB: 2023 Feb 28
Published in SASBDB:

SASDRX6 – lantibiotic dehydratase MadB (apo)

Thiopeptide-type bacteriocin biosynthesis domain containing protein experimental SAS data
GASBOR model
Sample: Thiopeptide-type bacteriocin biosynthesis domain containing protein dimer, 250 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 1 % (v/v) Glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
RgGuinier 4.5 nm
Dmax 13.6 nm
VolumePorod 440 nm3

SASDRY6 – lantibiotic dehydratase MadB bound to truncated maddinglicin with a full leader peptide (Leader3)

Thiopeptide-type bacteriocin biosynthesis domain containing proteinLantibiotic, gallidermin/nisin family experimental SAS data
GASBOR model
Sample: Thiopeptide-type bacteriocin biosynthesis domain containing protein dimer, 250 kDa Clostridium sp. Maddingley … protein
Lantibiotic, gallidermin/nisin family monomer, 3 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 1 % (v/v) Glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 3
RgGuinier 4.5 nm
Dmax 13.8 nm
VolumePorod 445 nm3