SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions.

Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV
J Mol Biol 429(5):667-687 (2017 Mar 10)
PMID: 28088481
doi: 10.1016/j.jmb.2017.01.008 		Submitted to SASBDB: 2016 Nov 4
Published in SASBDB:

SASDBG7 – Dps1, DNA binding protein under starvation conditions (SEC-SAXS)

DNA protection during starvation protein 1 experimental SAS data
GASBOR model
Sample: DNA protection during starvation protein 1 dodecamer, 276 kDa Deinococcus radiodurans R1 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
RgGuinier 4.2 nm
Dmax 12.8 nm
VolumePorod 437 nm3

SASDBH7 – Dps1 truncated, DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 1 experimental SAS data
GASBOR model
Sample: N-terminal truncated DNA protection during starvation protein 1 dodecamer, 216 kDa Deinococcus radiodurans R1 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
RgGuinier 3.9 nm
Dmax 10.0 nm
VolumePorod 291 nm3

SASDBF7 – Dps2, DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 2 experimental SAS data
EOM/RANCH model
Sample: N-terminal truncated DNA protection during starvation protein 2 dodecamer, 279 kDa Deinococcus radiodurans R1 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 Nov 22
RgGuinier 4.2 nm
Dmax 12.7 nm
VolumePorod 445 nm3