Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases

Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
Science Advances 9(31) (2023 Aug 04)

doi: 10.1126/sciadv.adh4721

Submitted to SASBDB: 2023 May 23 Published in SASBDB: 2024 May 30

SASDSA5 – dark-state short-linker LOV-activated diguanylate cyclase Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 May 4 RgGuinier 3.4 nm Dmax 13.6 nm VolumePorod 102 nm3

SASDSB5 – light-state short-linker LOV-activated diguanylate cyclase Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 May 4 RgGuinier 3.4 nm Dmax 15.7 nm VolumePorod 104 nm3

SASDSC5 – dark-state long-linker LOV-activated diguanylate cyclase Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 May 4 RgGuinier 2.9 nm Dmax 11.1 nm VolumePorod 115 nm3

SASDSD5 – light-state long-linker LOV-activated diguanylate cyclase Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2023 May 4 RgGuinier 3.5 nm Dmax 13.8 nm VolumePorod 123 nm3