Altered protein dynamics and a more reactive catalytic cysteine in a neurodegeneration-associated UCHL1 mutant.

Kenny S, Lai CH, Chiang TS, Brown K, Hewitt CS, Krabill AD, Chang HT, Wang YS, Flaherty DP, Danny Hsu ST, Das C
J Mol Biol :168438 (2024 Jan 5)
PMID: 38185323
doi: 10.1016/j.jmb.2024.168438 		Submitted to SASBDB: 2023 Oct 2
Published in SASBDB:

SASDTP2 – Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) wild type

Ubiquitin carboxyl-terminal hydrolase isozyme L1 experimental SAS data
Ubiquitin carboxyl-terminal hydrolase isozyme L1 Kratky plot
Sample: Ubiquitin carboxyl-terminal hydrolase isozyme L1 monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.03% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 Sep 2
RgGuinier 1.9 nm
Dmax 5.9 nm
VolumePorod 26 nm3

SASDTQ2 – Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) R178Q mutant

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (R178Q) experimental SAS data
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (R178Q) Kratky plot
Sample: Ubiquitin carboxyl-terminal hydrolase isozyme L1 (R178Q) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 0.03% NaN3, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 Sep 2
RgGuinier 1.9 nm
Dmax 5.8 nm
VolumePorod 25 nm3