B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations.

Wu CG, Balakrishnan VK, Merrill RA, Parihar PS, Konovolov K, Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y
Proc Natl Acad Sci U S A 121(1):e2310727120 (2024 Jan 2)

PMID: 38150499 doi: 10.1073/pnas.2310727120

Submitted to SASBDB: 2023 Oct 25 Published in SASBDB: 2023 Dec 8

SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) monomer, 70 kDa Homo sapiens protein Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20 RgGuinier 4.0 nm Dmax 12.0 nm VolumePorod 266 nm3

SASDTX2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme E198K mutant Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) monomer, 70 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20 RgGuinier 4.2 nm Dmax 14.0 nm VolumePorod 305 nm3