Structure of Pex8 in complex with peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation

Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M
(2025 Sep 02)

doi: 10.1101/2025.08.30.673231 		Submitted to SASBDB: 2025 Apr 25
Published in SASBDB:

SASDX84 – Peroxisomal targeting signal receptor Pex5 from P. pastoris

Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 15
RgGuinier 5.0 nm
Dmax 21.4 nm
VolumePorod 131 nm3

SASDX94 – Peroxisomal biogenesis factor 8 (Pex8) from P. pastoris

Peroxisomal biogenesis factor 8 experimental SAS data
MULTIFOXS model
Sample: Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
RgGuinier 3.6 nm
Dmax 13.0 nm
VolumePorod 117 nm3

SASDXA4 – Peroxisomal biogenesis factor 8 (Pex8) bound to Peroxisomal targeting signal receptor, Pex5 from P. pastoris

Peroxisomal targeting signal receptorPeroxisomal biogenesis factor 8 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein
Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
RgGuinier 5.1 nm
Dmax 23.5 nm
VolumePorod 247 nm3

SASDXB4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the N-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5NTD complex from P. pastoris)

Peroxisomal biogenesis factor 8Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Peroxisomal targeting signal receptor monomer, 32 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
RgGuinier 4.6 nm
Dmax 19.0 nm
VolumePorod 177 nm3

SASDXC4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the C-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5CTD complex from P. pastoris)

Peroxisomal biogenesis factor 8Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Peroxisomal targeting signal receptor monomer, 36 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 141 nm3