Structure of the aminoterminal domain of the birnaviral multifunctional VP3 protein and its unexplored critical role.

Ferrero DS, Gimenez MC, Sagar A, Rodríguez JM, Castón JR, Terebiznik MR, Bernadó P, Verdaguer N
PNAS Nexus 3(12):pgae521 (2024 Dec)

PMID: 39677362 doi: 10.1093/pnasnexus/pgae521

Submitted to SASBDB: 2024 Apr 19 Published in SASBDB: 2024 Oct 21

SASDUS6 – Amino-terminal domain of Infectious Bursal Disease Virus VP3 at pH8.0 Sample: Structural polyprotein dimer, 26 kDa Infectious bursal disease … protein Buffer: 50 mM Tris, 500 mM NaCl, 2 mM DTT, pH: 8 Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 27 RgGuinier 2.4 nm Dmax 10.0 nm VolumePorod 52 nm3

SASDUR7 – Infectious Bursal Disease Virus VP3 (central domains at pH 8.0) Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) monomer, 18 kDa Infectious bursal disease … protein Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT,, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2 RgGuinier 2.5 nm Dmax 10.0 nm VolumePorod 21 nm3

SASDUS7 – Infectious Bursal Disease Virus VP3 (C-terminal deleted at pH 8.0) Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) dimer, 55 kDa Infectious bursal disease … protein Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 1 RgGuinier 4.1 nm Dmax 15.0 nm VolumePorod 110 nm3