A hidden property of the iron-sulfur protein in the mononuclear iron-bound state: species-dependent structural ordering induced by magnetic fields.

Arai S, Soga S, Hirai M, Kobayashi R, Masai H, Kimura K, Maeda K, Nagashima H
FEBS J (2025 Dec 30)

PMID: 41466526 doi: 10.1111/febs.70377

Submitted to SASBDB: 2024 Jun 6 Published in SASBDB: 2026 Jan 2

SASDVB3 – Mononuclear iron bound form of pigeon ISCA1 under geomagnetic condition Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.7 nm Dmax 7.2 nm VolumePorod 39 nm3

SASDUF9 – Mononuclear iron bound form of pigeon ISCA1 under 180 mT magnetic field for 60min Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Jun 8 RgGuinier 2.8 nm Dmax 10.5 nm VolumePorod 85 nm3

SASDUG9 – Mononuclear iron bound form of human ISCA1 under geomagnetic field Sample: Human Iron-sulfur cluster assembly 1 homolog, mitochondrial, 14 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Apr 29 RgGuinier 4.1 nm Dmax 16.4 nm VolumePorod 105 nm3

SASDUJ9 – Mononuclear iron bound form of human ISCA1 under 180 mT magnetic field for 60 min Sample: Human Iron-sulfur cluster assembly 1 homolog, mitochondrial, 14 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Dec 1 RgGuinier 3.0 nm Dmax 11.2 nm VolumePorod 66 nm3

SASDVA3 – Iron-free pigeon ISCA1 under geomagnetic field Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Nov 1 RgGuinier 3.6 nm

SASDUK9 – Iron-free pigeon ISCA1 under 180mT magnetic field for 60 min Sample: Pigeon iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Nov 1 RgGuinier 3.9 nm

SASDUH9 – Mononuclear iron bound form of human ISCA1 under 180 mT magnetic field for 1 min Sample: Human Iron-sulfur cluster assembly 1 homolog, mitochondrial, 14 kDa Homo sapiens protein Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Dec 1 RgGuinier 2.9 nm Dmax 8.8 nm VolumePorod 55 nm3