Three-dimensional structure of the 3'X-tail of hepatitis C virus RNA in monomeric and dimeric states.

Cantero-Camacho Á, Fan L, Wang YX, Gallego J
RNA 23(9):1465-1476 (2017 Sep)

PMID: 28630140 doi: 10.1261/rna.060632.117

Submitted to SASBDB: 2016 Nov 23 Published in SASBDB: 2017 Jun 26

SASDBM7 – Subdomain SL1 of hepatitis C virus RNA domain 3'X at low ionic strength Sample: Subdomain SL1 of hepatitis C virus monomer, 15 kDa Hepatitis C virus RNA Buffer: 10mM Tris 0.1 mM EDTA, pH: 7 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11 RgGuinier 2.3 nm Dmax 8.1 nm

SASDBN7 – Subdomain SL2' of hepatitis C virus RNA domain 3'X at low ionic strength Sample: Subdomain SL2' of hepatitis C virus domain 3'X monomer, 18 kDa Hepatitis C virus RNA Buffer: 10mM Tris 0.1 mM EDTA, pH: 7 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11 RgGuinier 2.8 nm Dmax 10.0 nm

SASDBP7 – Domain 3'X of hepatitis C virus RNA at low ionic strength Sample: Domain 3'X of hepatitis C virus monomer, 32 kDa Hepatitis C virus RNA Buffer: 10mM Tris 0.1 mM EDTA, pH: 7 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11 RgGuinier 3.7 nm Dmax 14.8 nm

SASDBQ7 – Domain 3'X of hepatitis C virus RNA at higher ionic strength Sample: Domain 3'X of hepatitis C virus dimer, 63 kDa Hepatitis C virus RNA Buffer: 10mM Tris 0.1 mM EDTA 2 mM MgCl2 50 mM NaCl, pH: 7 Experiment: SAXS data collected at Rigaku BioSAXS-2000, on 2015 Nov 20 RgGuinier 6.0 nm Dmax 24.4 nm

SASDBR7 – Subdomain SL2' of hepatitis C virus RNA domain 3'X at higher ionic strength Sample: Subdomain SL2' of hepatitis C virus domain 3'X dimer, 36 kDa Hepatitis C virus RNA Buffer: 10mM Tris 0,1mM EDTA 2mM MgCl2, pH: 7 Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 11 RgGuinier 4.1 nm Dmax 16.9 nm