Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.

Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
Nat Commun 16(1):7970 (2025 Aug 26)

PMID: 40858555 doi: 10.1038/s41467-025-62922-4

Submitted to SASBDB: 2025 Jul 3 Published in SASBDB: 2025 Aug 19

SASDXX5 – SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.8 nm Dmax 17.1 nm

SASDXY5 – SARS-CoV-2 Nucleocapsid protein 1 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.6 nm Dmax 18.8 nm

SASDXZ5 – SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.7 nm Dmax 18.8 nm

SASDX26 – SARS-CoV-2 Nucleocapsid protein 3 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28 RgGuinier 6.3 nm Dmax 23.7 nm

SASDX36 – SARS-CoV-2 Nucleocapsid protein 4 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28 RgGuinier 6.6 nm Dmax 22.5 nm

SASDX46 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.6 nm Dmax 15.0 nm

SASDX56 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.4 nm Dmax 166.0 nm

SASDX66 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.6 nm Dmax 19.3 nm

SASDX76 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 2 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18 RgGuinier 5.5 nm Dmax 17.8 nm

SASDX86 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 3 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28 RgGuinier 5.9 nm Dmax 19.8 nm

SASDX96 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 4 mg/mL Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5 Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28 RgGuinier 5.9 nm Dmax 23.5 nm