Structural characterization of the interaction between BRCA1-BARD1 and RNA polymerase II

Tomas Klumpler

Submitted to SASBDB: 2024 Oct 18 Published in SASBDB: 2026 Oct 18

SASDV28 – Breast cancer type 1 susceptibility protein, BRCA1 C-terminus domain (2 mg/ml) Sample: Breast cancer type 1 susceptibility protein, 25 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.3 nm Dmax 5.9 nm VolumePorod 34 nm3

SASDV38 – Breast cancer type 1 susceptibility protein, BRCA1 C-terminus domain (3 mg/ml) Sample: Breast cancer type 1 susceptibility protein, 25 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.5 nm Dmax 6.8 nm VolumePorod 36 nm3

SASDV48 – Breast cancer type 1 susceptibility protein, BRCA1 C-terminus domain (4 mg/ml) Sample: Breast cancer type 1 susceptibility protein, 25 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.7 nm Dmax 7.8 nm VolumePorod 44 nm3

SASDV58 – BRCA1-associated RING domain protein 1, BRCA1 C-terminus domain (2 mg/ml) Sample: Isoform alpha of BRCA1-associated RING domain protein 1, 26 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.3 nm Dmax 6.0 nm VolumePorod 37 nm3

SASDV68 – BRCA1-associated RING domain protein 1, BRCA1 C-terminus domain (3 mg/ml) Sample: Isoform alpha of BRCA1-associated RING domain protein 1, 26 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.6 nm Dmax 6.5 nm VolumePorod 36 nm3

SASDV78 – BRCA1-associated RING domain protein 1, BRCA1 C-terminus domain (4 mg/ml) Sample: Isoform alpha of BRCA1-associated RING domain protein 1, 26 kDa Homo sapiens protein Buffer: 25 mM Tris, 200 mM NaCl, 1 mM DTT, pH: 7.9 Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Dec 19 RgGuinier 2.7 nm Dmax 7.5 nm VolumePorod 37 nm3