Structure of relaxase from Bacillus subtilis conjugative plasmid pLS20 reveals a new mechanism of DNA processing mediated by dimerization

SASDX82 – N-terminal domain of relaxase from Bacillus subtilis conjugative plasmid pLS20 Sample: Relaxase monomer, 29 kDa Bacillus subtilis subsp. … protein Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3 RgGuinier 2.3 nm Dmax 7.5 nm VolumePorod 48 nm3

SASDX92 – N-terminal domain of relaxase from Bacillus subtilis conjugative plasmid pLS20 in complex with nic sequence DNA Sample: Relaxase dimer, 57 kDa Bacillus subtilis subsp. … protein Nic sequence, single stranded dimer, 12 kDa Bacillus subtilis DNA Buffer: 20 mM Tris, 300 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3 RgGuinier 2.5 nm Dmax 8.2 nm VolumePorod 50 nm3