Solution Structure Determination and Biophysical Studies of 7SK RNP and 7SL SRP RNAs.

D'Souza MH, Pereira HS, Tersteeg S, Vasudeva G, Siddiqui MQ, Agunlejika T, Kerr L, Girodat D, Patel T
RNA (2025 Jul 31)

PMID: 40744708 doi: 10.1261/rna.080414.125

Submitted to SASBDB: 2024 Nov 7 Published in SASBDB: 2025 Aug 3

SASDVC9 – 7SK small nuclear RNA State A Low Magnesium Concentration Ensemble Sample: RNA component of 7SK nuclear ribonucleoprotein monomer, 107 kDa Homo sapiens RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH: 5.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 21 RgGuinier 8.0 nm Dmax 23.2 nm VolumePorod 600 nm3

SASDVD9 – 7SK small nuclear RNA State B Low Magnesium Concentration Ensemble Sample: RNA component of 7SK nuclear ribonucleoprotein monomer, 107 kDa Homo sapiens RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH: 5.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 21 RgGuinier 7.9 nm Dmax 24.1 nm VolumePorod 290 nm3

SASDVE9 – 7SL Signal Recognition Particle Sample: 7SL Signal Reocgnition Particle RNA monomer, 98 kDa Homo sapiens RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 15 mM KCl, 5% Glycerol, 3 mM MgCl2, pH: 5.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 21 RgGuinier 6.7 nm Dmax 21.7 nm VolumePorod 300 nm3