Allergen-induced structural rearrangements in IgE: insights from SAXS and molecular dynamics.

Gómez-Velasco H, García-Ramírez B, Siliqi D, Graewert MA, Quintero-Martinez A, Ortega E, Rodríguez-Romero A
Int J Biol Macromol :147658 (2025 Sep 13)

PMID: 40953619 doi: 10.1016/j.ijbiomac.2025.147658

Submitted to SASBDB: 2025 May 7 Published in SASBDB: 2025 Sep 17

SASDVF9 – Mouse complex murine Immunoglobulin E (IgE) antibody with profilin Sample: Murine Immunoglobulin E (IgE) antibodies monomer, 165 kDa Mus musculus protein Profilin-2 monomer, 18 kDa Hevea brasiliensis protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3 RgGuinier 6.0 nm Dmax 19.9 nm VolumePorod 504 nm3

SASDVG9 – Mouse murine Immunoglobulin E (IgE) antibodies Sample: Murine Immunoglobulin E (IgE) antibodies monomer, 165 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3 RgGuinier 5.5 nm Dmax 19.2 nm VolumePorod 422 nm3

SASDVH9 – allergen Hev b 8 protein, Profilin-2 (TAG) Sample: Profilin-2 monomer, 18 kDa Hevea brasiliensis protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Oct 3 RgGuinier 2.0 nm Dmax 7.4 nm VolumePorod 30 nm3

SASDXH4 – Mouse complex murine Immunoglobulin E (IgE) antibody with profilin (without HisTAG) Sample: Murine Immunoglobulin E (IgE) antibodies monomer, 165 kDa Mus musculus protein Profilin-2 monomer, 18 kDa Hevea brasiliensis protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Apr 11 RgGuinier 5.7 nm Dmax 19.9 nm VolumePorod 450 nm3

SASDXK4 – Mouse murine Immunoglobulin E (IgE) antibody Sample: Murine Immunoglobulin E (IgE) antibodies monomer, 165 kDa Mus musculus protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Apr 11 RgGuinier 5.2 nm Dmax 16.3 nm VolumePorod 420 nm3

SASDXJ4 – Allergen Hev b 8 protein, Profilin-2 Sample: Profilin-2 monomer, 18 kDa Hevea brasiliensis protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 8.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2025 Apr 11 RgGuinier 1.7 nm Dmax 4.5 nm VolumePorod 21 nm3