Hierarchy in regulator interactions with distant transcriptional activation domains empowers rheostatic regulation

Due A, Davey N, Thomasen F, Morffy N, Prestel A, Brakti I, O'Shea C, Strader L, Lindorff‐Larsen K, Skriver K, Kragelund B
Protein Science 34(6) (2025 May 15)

doi: 10.1002/pro.70142 		Submitted to SASBDB: 2024 Nov 11
Published in SASBDB:

SASDVN9 – NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) from A.thaliana in unbound state at pH 7.0

NAC domain-containing protein 46 experimental SAS data
NAC domain-containing protein 46 Kratky plot
Sample: NAC domain-containing protein 46 monomer, 18 kDa Arabidopsis thaliana protein
Buffer: 20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Dec 1
RgGuinier 3.5 nm
Dmax 16.5 nm
VolumePorod 31 nm3

SASDVP9 – NAC domain-containing protein 46 (ANAC046) Intrinsically disordered region (172-338) in a 1:1 molar ratio binding with Inactive poly [ADP-ribose] polymerase RCD1-RST (499-572) from A.thaliana at pH 7.0

NAC domain-containing protein 46Inactive poly [ADP-ribose] polymerase RCD1 experimental SAS data
NAC domain-containing protein 46 Inactive poly [ADP-ribose] polymerase RCD1 Kratky plot
Sample: NAC domain-containing protein 46 monomer, 18 kDa Arabidopsis thaliana protein
Inactive poly [ADP-ribose] polymerase RCD1 monomer, 9 kDa Arabidopsis thaliana protein
Buffer: 20mM Na2HPO4/NaH2PO4, 100mM NaCl, 5mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Dec 1
RgGuinier 3.8 nm
Dmax 20.6 nm
VolumePorod 38 nm3