Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin

Yang W, Kim S, Kim M, Shin H, Lee J, Sandmann A, Park O, Dissmeyer N, Song H
Nature Communications 16(1) (2025 Aug 21)

doi: 10.1038/s41467-025-63282-9 		Submitted to SASBDB: 2024 Nov 26
Published in SASBDB:

SASDWM2 – Wild-type E3 ubiquitin-protein ligase PRT1 (Proteolysis1)

E3 ubiquitin-protein ligase PRT1 experimental SAS data
DAMMIF model
Sample: E3 ubiquitin-protein ligase PRT1 monomer, 46 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 2% w/v glycerol, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 22
RgGuinier 3.4 nm
Dmax 12.7 nm
VolumePorod 61 nm3