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SAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation

Submitted to SASBDB: 2025 Feb 19
Published in SASBDB:

SASDWD8 – Full-length G3BP1 (residues 1-466) at pH 7.5, 150 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Dec 13
RgGuinier 6.6 nm
Dmax 32.4 nm
VolumePorod 221 nm3

SASDWE8 – Full-length G3BP1 (residues 1-466) at pH 7.5, 50 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 2
RgGuinier 5.8 nm
Dmax 28.7 nm
VolumePorod 218 nm3

SASDWF8 – Full-length G3BP1 (residues 1-466) at pH 6.0, 50 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: 50 mM MES, 50 mM NaCl,, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 2
RgGuinier 4.9 nm
Dmax 22.5 nm
VolumePorod 163 nm3

SASDWG8 – NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

NTF2L domain of Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIF model
Sample: NTF2L domain of Ras GTPase-activating protein-binding protein 1 dimer, 32 kDa protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 2
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDWH8 – NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 7.5, 150 mM NaCl

NTF2L domain and IDR1 of stress granule core protein G3BP1 experimental SAS data
DAMMIF model
Sample: NTF2L domain and IDR1 of stress granule core protein G3BP1 dimer, 53 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Dec 4
RgGuinier 4.0 nm
Dmax 16.2 nm
VolumePorod 108 nm3

SASDWJ8 – NTF2L domain and acidic IDR1 region of G3BP1 (NTF2LA, residues 1-232) at pH 6.0, 50 mM NaCl

NTF2L domain and IDR1 of stress granule core protein G3BP1 experimental SAS data
DAMMIF model
Sample: NTF2L domain and IDR1 of stress granule core protein G3BP1 dimer, 53 kDa Homo sapiens protein
Buffer: 50 mM MES, 50 mM NaCl,, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Dec 4
RgGuinier 3.6 nm
Dmax 15.1 nm
VolumePorod 96 nm3

SASDWK8 – G3BP1ΔRGG (residues 1-413) at pH 7.5, 150 mM NaCl

RGG deletion mutant of Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: RGG deletion mutant of Ras GTPase-activating protein-binding protein 1 dimer, 94 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Dec 4
RgGuinier 6.6 nm
Dmax 32.0 nm
VolumePorod 226 nm3

SASDWL8 – G3BP1ΔRGG (residues 1-413) at pH 6, 50 mM NaCl

RGG deletion mutant of Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: RGG deletion mutant of Ras GTPase-activating protein-binding protein 1 dimer, 94 kDa Homo sapiens protein
Buffer: 50 mM MES, 50 mM NaCl,, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Dec 4
RgGuinier 6.0 nm
Dmax 31.0 nm
VolumePorod 213 nm3