AF4-to-SAXS: Expanded Characterization of Nanoparticles and Proteins at the P12 Biosaxs Beamline

Melissa Graewert

Submitted to SASBDB: 2024 Dec 31 Published in SASBDB: 2025 Jun 7

SASDW94 – Horse apoferritin from AF4-SAXS measurements Sample: Ferritin light chain 24-mer, 480 kDa Equus caballus protein Buffer: phosphate buffered saline, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 5.4 nm Dmax 13.3 nm VolumePorod 632 nm3

SASDWA4 – Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13 RgGuinier 3.4 nm Dmax 9.5 nm VolumePorod 197 nm3

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein Buffer: phosphate buffered saline, 1% glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13 RgGuinier 4.2 nm Dmax 12.6 nm VolumePorod 297 nm3

SASDWC4 – 100 nm polystyrene beads from AF4-SAXS measurements Sample: Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 47.7 nm Dmax 111.0 nm

SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements Sample: Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct Buffer: water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19 RgGuinier 9.0 nm Dmax 25.0 nm