Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination

Rinaldi F, Franco P, Veronesi M, Romeo E, Bresciani V, Varignani G, Catalano F, Bernetti M, Masetti M, Langer J, Girotto S, Cavalli A
(2025 Nov 13)

doi: 10.1101/2025.11.13.688182

Submitted to SASBDB: 2025 Jan 24 Published in SASBDB: 2026 Jan 24

SASDX95 – DNA repair protein RAD51 homolog 1 (RAD51) [F86E, A89E] in complex with Breast cancer type 2 susceptibility protein (BRCA2) truncation containing the second, third and fourth BRC repeats (BRC2-4) at 1 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 2-4) monomer, 39 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) trimer, 111 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 6.5 nm Dmax 28.5 nm VolumePorod 334 nm3

SASDXA5 – DNA repair protein RAD51 homolog 1 (RAD51) [F86E, A89E] in complex with Breast cancer type 2 susceptibility protein (BRCA2) truncation containing the second, third and fourth BRC repeats (BRC2-4) at 2 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 2-4) monomer, 39 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) trimer, 111 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 6.5 nm Dmax 28.5 nm VolumePorod 473 nm3

SASDXB5 – DNA repair protein RAD51 homolog 1 (RAD51) [F86E, A89E] in complex with Breast cancer type 2 susceptibility protein (BRCA2) truncation containing the second, third and fourth BRC repeats (BRC2-4) at 3.5 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 2-4) monomer, 39 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) trimer, 111 kDa Homo sapiens protein Buffer: 20 mM Hepes, 350 mM KCl, 1 mM DTT, 5% Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 4 RgGuinier 6.9 nm Dmax 28.5 nm VolumePorod 652 nm3

SASDXC5 – DNA repair protein RAD51 homolog 1 (RAD51) [F86E, A89E] in complex with Breast cancer type 2 susceptibility protein (BRCA2) truncation containing the second, third and fourth BRC repeats (BRC2-4) at 7 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 2-4) monomer, 39 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) trimer, 111 kDa Homo sapiens protein Buffer: 20 mM Hepes, 350 mM KCl, 1 mM DTT, 5% Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 4 RgGuinier 6.8 nm Dmax 28.3 nm VolumePorod 542 nm3

SASDWT5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) SEC-SAXS Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2 RgGuinier 2.0 nm Dmax 6.6 nm VolumePorod 57 nm3

SASDWU5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 1 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.0 nm Dmax 6.3 nm VolumePorod 51 nm3

SASDWV5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 2 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.1 nm Dmax 6.6 nm VolumePorod 49 nm3

SASDWW5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 4 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.1 nm Dmax 6.3 nm VolumePorod 57 nm3

SASDWX5 – N-terminal truncated RAD51 in complex with fourth BRC repeat (BRC4) at 6 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (Δ97) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.1 nm Dmax 6.6 nm VolumePorod 63 nm3

SASDWY5 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) SEC-SAXS Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2 RgGuinier 2.8 nm Dmax 10.5 nm VolumePorod 74 nm3

SASDWZ5 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) at 0.5 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.8 nm Dmax 11.0 nm VolumePorod 67 nm3

SASDW26 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) at 1 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.7 nm Dmax 10.0 nm VolumePorod 64 nm3

SASDW36 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) at 2 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.7 nm Dmax 10.3 nm VolumePorod 66 nm3

SASDW46 – RAD51 [F86E, A89E] in complex with fourth BRC repeat (BRC4) at 4 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 4) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.8 nm Dmax 10.0 nm VolumePorod 72 nm3

SASDW56 – RAD51 [F86E, A89E] in complex with second BRC repeat (BRC2) SEC-SAXS Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 2) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2 RgGuinier 2.7 nm Dmax 9.6 nm VolumePorod 67 nm3

SASDW66 – RAD51 [F86E, A89E] in complex with second BRC repeat (BRC2) at 0.5 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 2) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.6 nm Dmax 9.5 nm VolumePorod 83 nm3

SASDW76 – RAD51 [F86E, A89E] in complex with second BRC repeat (BRC2) at 1 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 2) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.7 nm Dmax 9.6 nm VolumePorod 63 nm3

SASDW86 – RAD51 [F86E, A89E] in complex with second BRC repeat (BRC2) at 2 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeat 2) monomer, 4 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 2.7 nm Dmax 9.8 nm VolumePorod 69 nm3

SASDW96 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the second and the third BRC repeats (BRC2-3) SEC-SAXS Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 2-3) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 100 mM Li₂SO₄, 1 mM DTT, 1% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.5 nm Dmax 23.0 nm VolumePorod 167 nm3

SASDWA6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the second and the third BRC repeats (BRC2-3) at 0.5 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 2-3) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 6.0 nm Dmax 21.0 nm VolumePorod 236 nm3

SASDWB6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the second and the third BRC repeats (BRC2-3) at 1 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 2-3) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.7 nm Dmax 23.0 nm VolumePorod 225 nm3

SASDWC6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the second and the third BRC repeats (BRC2-3) at 2 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 2-3) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.7 nm Dmax 22.0 nm VolumePorod 167 nm3

SASDWD6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the second, third and fourth BRC repeats (BRC2-4) SEC-SAXS Sample: Breast cancer type 2 susceptibility protein (BRC repeats 2-4) monomer, 39 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) trimer, 111 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 100 mM Li₂SO₄, 1 mM DTT, 1% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 6.7 nm Dmax 28.0 nm VolumePorod 259 nm3

SASDWE6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the third and fourth BRC repeats (BRC3-4) SEC-SAXS Sample: Breast cancer type 2 susceptibility protein (BRC repeats 3-4) monomer, 16 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2 RgGuinier 4.6 nm Dmax 18.0 nm VolumePorod 142 nm3

SASDWF6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the third and fourth BRC repeats (BRC3-4) at 0.5 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 3-4) monomer, 16 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 4.6 nm Dmax 18.5 nm VolumePorod 175 nm3

SASDWG6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the third and fourth BRC repeats (BRC3-4) at 1 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 3-4) monomer, 16 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 4.5 nm Dmax 18.0 nm VolumePorod 140 nm3

SASDWH6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the third and fourth BRC repeats (BRC3-4) at 2 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 3-4) monomer, 16 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 4.6 nm Dmax 18.3 nm VolumePorod 148 nm3

SASDWJ6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first and the second BRC repeats (BRC1-2) SEC-SAXS Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 1-2) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 1 mM DTT, 2% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 May 2 RgGuinier 5.3 nm Dmax 21.0 nm VolumePorod 165 nm3

SASDWK6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first and the second BRC repeats (BRC1-2) at 0.5 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 1-2) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.7 nm Dmax 24.0 nm VolumePorod 249 nm3

SASDWL6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first and the second BRC repeats (BRC1-2) at 1 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 1-2) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.5 nm Dmax 21.0 nm VolumePorod 208 nm3

SASDWM6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first and the second BRC repeats (BRC1-2) at 2 mg/mL Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) dimer, 74 kDa Homo sapiens protein Breast cancer type 2 susceptibility protein (BRC repeats 1-2) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jul 1 RgGuinier 5.5 nm Dmax 23.0 nm VolumePorod 191 nm3

SASDWN6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first, second, third and fourth BRC repeats (BRC1-4) SEC-SAXS Sample: Breast cancer type 2 susceptibility protein (BRC repeats 1-4) monomer, 62 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) tetramer, 148 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 100 mM Li₂SO₄, 1 mM DTT, 1% sucrose, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 7.7 nm Dmax 34.0 nm VolumePorod 409 nm3

SASDWP6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first, second, third and fourth BRC repeats (BRC1-4) at 0.5 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 1-4) monomer, 62 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) tetramer, 148 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 8.1 nm Dmax 33.0 nm VolumePorod 422 nm3

SASDWQ6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first, second, third and fourth BRC repeats (BRC1-4) at 0.98 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 1-4) monomer, 62 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) tetramer, 148 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 7.6 nm Dmax 33.3 nm VolumePorod 392 nm3

SASDWR6 – RAD51 [F86E, A89E] in complex with BRCA2 truncation containing the first, second, third and fourth BRC repeats (BRC1-4) at 2 mg/mL Sample: Breast cancer type 2 susceptibility protein (BRC repeats 1-4) monomer, 62 kDa Homo sapiens protein DNA repair protein RAD51 homolog 1 (F86E A89E) tetramer, 148 kDa Homo sapiens protein Buffer: 20 mM K₂HPO₄/KH₂PO₄, 100 mM NaCl, 200 mM Li₂SO₄, 5% glycerol, 1 mM DTT, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Dec 2 RgGuinier 7.7 nm Dmax 33.0 nm VolumePorod 417 nm3