Structural Dynamics of Dengue Virus UTRs and Their Cyclization.

Robinson ZE, Pereira HS, D'Souza MH, Patel TR
Biophys J (2025 Sep 5)

PMID: 40914810 doi: 10.1016/j.bpj.2025.09.004

Submitted to SASBDB: 2025 Apr 10 Published in SASBDB: 2025 Sep 9

SASDXT3 – Dengue Virus 2 (DENV2) RNA, 5' Untranslated region Sample: DENV2 RNA, 5' Untranslated region monomer, 57 kDa RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 8 RgGuinier 3.7 nm Dmax 11.5 nm VolumePorod 66 nm3

SASDXC3 – Dengue Virus 2 (DENV2) 5' UTR Sample: DENV2 5' untranslated terminal region monomer, 57 kDa RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 10 RgGuinier 3.7 nm Dmax 11.5 nm VolumePorod 57 nm3

SASDXD3 – Dengue Virus 2 (DENV2) 3' UTR Sample: DENV2 3' untranslated region monomer, 160 kDa RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 10 RgGuinier 6.0 nm Dmax 18.7 nm VolumePorod 155 nm3

SASDXE3 – Dengue Virus 2 (DENV2) complex 5'-3' Sample: DENV2 complex 5'-3' monomer, 217 kDa RNA Buffer: 10 mM Bis-Tris, 100 mM NaCl, 5 mM MgCl2, 15 mM KCl, 5% glycerol, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Sep 10 RgGuinier 7.5 nm Dmax 22.0 nm VolumePorod 320 nm3