Differentiating the solution structures and stability of transthyretin tetramer complexed with tolcapone and tafamidis using SEC-SWAXS and NMR.

Shih O, Feng YC, Agrawal S, Liao KF, Yeh YQ, Chang JW, Yu TY, Jeng US
J Appl Crystallogr 58(Pt 4):1373-1383 (2025 Aug 1)

PMID: 40765976 doi: 10.1107/S1600576725004716

Submitted to SASBDB: 2025 Apr 13 Published in SASBDB: 2025 Apr 24

SASDXW3 – Transthyretin tetramer Sample: Transthyretin tetramer, 55 kDa Homo sapiens protein Buffer: 20 mM Tris, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Sep 4 RgGuinier 2.4 nm Dmax 7.2 nm VolumePorod 92 nm3

SASDXX3 – Transthyretin tetramer with over-saturated tolcapone Sample: Transthyretin tetramer, 55 kDa Homo sapiens protein Tolcapone dimer, 1 kDa Buffer: 20 mM Tris, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 19 RgGuinier 2.5 nm Dmax 8.6 nm VolumePorod 107 nm3

SASDXY3 – Transthyretin tetramer with over-saturated tafamidis Sample: Transthyretin tetramer, 55 kDa Homo sapiens protein Tafamidis dimer, 1 kDa Buffer: 20 mM Tris, 50 mM NaCl, pH: 7 Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 19 RgGuinier 2.5 nm Dmax 9.0 nm VolumePorod 69 nm3