Molecular Insights into CLD Domain Dynamics and Toxin Recruitment of the HlyA E. coli T1SS

Gentile R, Schott-Verdugo S, Khosa S, Günes C, Bonus M, Reiners J, Smits S, Schmitt L, Gohlke H
Journal of Molecular Biology :169485 (2025 Oct)

doi: 10.1016/j.jmb.2025.169485 		Submitted to SASBDB: 2025 May 22
Published in SASBDB:

SASDXZ4 – Hemolysin A Type 1 Secretion System (Hly T1SS)

Alpha-hemolysin translocation ATP-binding protein HlyBHlyA (GFP-Hemolysin with Flag-Tag insertion)Membrane fusion protein (MFP) family protein (R14C; L229V)Outer membrane protein TolC experimental SAS data
DAMMIF model
Sample: Alpha-hemolysin translocation ATP-binding protein HlyB hexamer, 480 kDa Escherichia coli (strain … protein
HlyA (GFP-Hemolysin with Flag-Tag insertion) monomer, 144 kDa Escherichia coli protein
Membrane fusion protein (MFP) family protein (R14C; L229V) hexamer, 327 kDa Escherichia coli (strain … protein
Outer membrane protein TolC trimer, 161 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.0063% glyco-diosgenin (GDN), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Jun 29
RgGuinier 11.4 nm
Dmax 46.2 nm
VolumePorod 2837 nm3