Molecular basis of Fab-dependent IgA antibody recognition by gut-associated metallopeptidases

María Ángeles Márquez-Moñino

Submitted to SASBDB: 2025 Jun 3 Published in SASBDB: 2025 Jul 4

SASDX45 – IgA protease (ThomasA) 31-1167 Sample: IgA protease monomer, 127 kDa Thomasclavelia ramosa protein Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19 RgGuinier 6.4 nm Dmax 25.0 nm VolumePorod 208 nm3

SASDX55 – IgA protease (ThomasA) 31-878 Sample: IgA protease monomer, 96 kDa Thomasclavelia ramosa protein Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19 RgGuinier 3.4 nm Dmax 10.6 nm VolumePorod 101 nm3

SASDX65 – IgA protease (ThomasA) 323-878 Sample: IgA protease monomer, 64 kDa Thomasclavelia ramosa protein Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19 RgGuinier 3.0 nm Dmax 9.2 nm VolumePorod 72 nm3