Molecular basis of Fab-dependent IgA antibody recognition by gut-bacterial metallopeptidases

Márquez-Moñino M, Martínez Gascueña A, Azzam T, Persson A, Manzanares-Gomez A, Aguillo-Urarte M, Brown T, Montero-Sagarminaga A, Lood R, Naegeli A, Connell S, Sastre D, Sundberg E, Trastoy B
The EMBO Journal (2025 Jul 31)

doi: 10.1038/s44318-025-00518-w 		Submitted to SASBDB: 2025 Jun 3
Published in SASBDB:

SASDX45 – IgA protease (ThomasA) 31-1167

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 127 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
RgGuinier 6.4 nm
Dmax 25.0 nm
VolumePorod 208 nm3

SASDX55 – IgA protease (ThomasA) 31-878

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 96 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 101 nm3

SASDX65 – IgA protease (ThomasA) 323-878

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 64 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
RgGuinier 3.0 nm
Dmax 9.2 nm
VolumePorod 72 nm3