Molecular architecture of the human citrate synthase–malate dehydrogenase 2 metabolon

Kayll A, Rupakheti U, St John R, Rementeria G, Provost J, Berndsen C
Acta Crystallographica Section D Structural Biology 82(7):740-750 (2026 Jun 21)

doi: 10.1107/S2059798326005802 		Submitted to SASBDB: 2025 Aug 1
Published in SASBDB:

SASDX87 – human Citrate Synthase

citrate synthase experimental SAS data
OTHER model
Sample: Citrate synthase dimer, 102 kDa Homo sapiens protein
Buffer: 50 mM Tris, 100 mM NaCl, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Jun 25
RgGuinier 3.1 nm
Dmax 9.6 nm
VolumePorod 113 nm3

SASDX97 – human Citrate Synthase

citrate synthase experimental SAS data
OTHER model
Sample: Citrate synthase dimer, 102 kDa Homo sapiens protein
Buffer: 50 mM Tris, 100 mM NaCl, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Jun 25
RgGuinier 3.1 nm
Dmax 10.6 nm
VolumePorod 131 nm3

SASDXA7 – hexamer of human citrate synthase and human malate dehydrogenase 2

citrate synthaseMalate dehydrogenase, mitochondrialMalate dehydrogenase, mitochondrial experimental SAS data
OTHER model
Sample: Citrate synthase dimer, 102 kDa Homo sapiens protein
Malate dehydrogenase, mitochondrial dimer, 70 kDa Homo sapiens protein
Malate dehydrogenase, mitochondrial dimer, 70 kDa Homo sapiens protein
Buffer: 50 mM Tris, 100 mM NaCl, 0.1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2024 Jun 25
RgGuinier 4.4 nm
Dmax 15.3 nm
VolumePorod 266 nm3