Small angle x-ray scattering analysis of PE25, PPE41, EspG5 and EccA5 N-terminal domain complex of ESX-5 pathway of Mycobacterium tuberculosis

Rajlakshmi K

Submitted to SASBDB: 2025 Aug 5 Published in SASBDB: 2026 Aug 5

SASDXD7 – Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 secretion-associated protein pathway of Mycobacterium tuberculosis Sample: PE-PGRS family protein PE25 monomer, 9 kDa Mycobacterium tuberculosis (strain … protein PPE family protein PPE41 monomer, 20 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion-associated protein EspG5 monomer, 32 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion system protein EccA5 monomer, 31 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50 mM HEPES, 200 mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2025 May 9 RgGuinier 4.6 nm Dmax 13.7 nm VolumePorod 140 nm3

SASDXE7 – Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 pathway of Mycobacterium tuberculosis Sample: PE-PGRS family protein PE25 monomer, 9 kDa Mycobacterium tuberculosis (strain … protein PPE family protein PPE41 monomer, 20 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion-associated protein EspG5 monomer, 32 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion system protein EccA5 monomer, 31 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50mM HEPES, 200mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2025 May 8 RgGuinier 4.6 nm Dmax 13.7 nm VolumePorod 141 nm3

SASDXF7 – Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 pathway of Mycobacterium tuberculosis Sample: PE-PGRS family protein PE25 monomer, 9 kDa Mycobacterium tuberculosis (strain … protein PPE family protein PPE41 monomer, 20 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion-associated protein EspG5 monomer, 32 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion system protein EccA5 monomer, 31 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50mM Hepes 200mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2025 May 8 RgGuinier 4.6 nm Dmax 13.7 nm VolumePorod 140 nm3

SASDXG7 – Complex of PE25, PPE41, EspG5 and EccA5 N-terminal domain of ESX-5 pathway of Mycobacterium tuberculosis Sample: ESX-5 secretion-associated protein EspG5 monomer, 32 kDa Mycobacterium tuberculosis (strain … protein ESX-5 secretion system protein EccA5 monomer, 31 kDa Mycobacterium tuberculosis (strain … protein PPE family protein PPE41 monomer, 20 kDa Mycobacterium tuberculosis (strain … protein PE-PGRS family protein PE25 monomer, 9 kDa Mycobacterium tuberculosis (strain … protein Buffer: 50mM HEPES, 200mM NaCl, pH: 8 Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2025 May 9 RgGuinier 4.6 nm Dmax 15.0 nm VolumePorod 104 nm3