Structures of Nucleotide-Bound Redondovirus Rep Protein Link Conformation and Function

Kushol Gupta

Submitted to SASBDB: 2025 Aug 11 Published in SASBDB: 2026 Feb 16

SASDXP7 – Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses Sample: Human lung-associated vientovirus FB Replication-Associated Protein Dodecamer dodecamer, 487340 kDa Vientovirus FB protein Buffer: 20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jan 25 RgGuinier 5.2 nm Dmax 155.0 nm

SASDXQ7 – Human lung-associated vientovirus FB Replication-Associated Protein Apo form (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses Sample: Human lung-associated vientovirus FB Replication-Associated Protein Hexamer hexamer, 243670 kDa Vitentovirus RB protein Buffer: 20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jan 25 RgGuinier 4.7 nm Dmax 144.0 nm VolumePorod 488 nm3

SASDXR7 – Human lung-associated vientovirus FB Replication-Associated Protein ATP-bound (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses Sample: Human lung-associated vientovirus FB Replication-Associated Protein Dodecamer dodecamer, 487340 kDa Vientovirus FB protein Buffer: 20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jan 25 RgGuinier 5.5 nm Dmax 162.0 nm VolumePorod 668 nm3

SASDXS7 – Human lung-associated vientovirus FB Replication-Associated Protein ADP-bound (Rep) Dodecamer from SEC-SAXS with SVD-EFA analyses Sample: Human lung-associated vientovirus FB Replication-Associated Protein Dodecamer dodecamer, 487340 kDa Vientovirus FB protein Buffer: 20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jan 25 RgGuinier 5.5 nm Dmax 239.0 nm VolumePorod 850 nm3

SASDXT7 – Human lung-associated vientovirus FB Replication-Associated Protein ADP-bound (Rep) Hexamer from SEC-SAXS with SVD-EFA analyses Sample: Human lung-associated vientovirus FB Replication-Associated Protein Hexamer hexamer, 243670 kDa Vitentovirus RB protein Buffer: 20 mM HEPES, 300 mM NaCl, 0.1 mM TCEP, pH: 7.5 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jan 25 RgGuinier 4.9 nm Dmax 162.0 nm VolumePorod 487 nm3