Hexahistidine-tagged QseC bound to operator DNA sites

Joshua Ramsay Submitted to SASBDB: 2025 Aug 27
Published in SASBDB:

SASDXJ8 – Hexahistidine-tagged Quorum sensing controller protein (QseC) bound to dual operator OL and OR DNA sites

Quorum sensing controller protein QseCQseC Operator OL+OR DNA experimental SAS data
DAMMIN model
Sample: Quorum sensing controller protein QseC tetramer, 44 kDa Mesorhizobium japonicum protein
QseC Operator OL+OR DNA monomer, 24 kDa DNA
Buffer: 50mM Tris, 300mM NaCl, 2.5% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2020 Apr 24
RgGuinier 3.2 nm
Dmax 12.6 nm
VolumePorod 70 nm3

SASDXK8 – Hexahistidine-tagged Quorum sensing controller protein (QseC) bound to single operator OL DNA sites

Quorum sensing controller protein QseCQseC Operator OL DNA experimental SAS data
DAMFILT model
Sample: Quorum sensing controller protein QseC dimer, 22 kDa Mesorhizobium japonicum protein
QseC Operator OL DNA monomer, 24 kDa DNA
Buffer: 50mM Tris, 300mM NaCl, 2.5% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2020 Apr 24
RgGuinier 3.5 nm
Dmax 15.4 nm
VolumePorod 53 nm3