The BRCT domain enhances DNA binding and catalytic efficiency of fungal PARPs.

Bainbridge AG, Gianga TM, Hussain R, Parkin C, Longhurst AD, Helmstetter N, Farrer RA, Catania S, Siligardi G, Rack JGM
Nat Commun 17(1) (2026 Jul 24)

PMID: 42642413 doi: 10.1038/s41467-026-75844-6

Submitted to SASBDB: 2025 Sep 22 Published in SASBDB: 2026 Aug 27

SASDXE9 – poly(ADP-ribose)polymerase 1 (Af-PARP1) apo protein Sample: Aspergillus fumigatus poly(ADP-ribose)polymerase 1 monomer, 74 kDa Aspergillus fumigatus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, 2.5% (v/v) Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Nov 14 RgGuinier 4.4 nm Dmax 13.7 nm VolumePorod 131 nm3

SASDXF9 – poly(ADP-ribose)polymerase 1 (Af-PARP1) with N-terminal dBRCT domain truncation Sample: Aspergillus fumigatus poly(ADP-ribosyl)polymerase 1 dBRCT monomer, 58 kDa Aspergillus fumigatus protein Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, 2.5% (v/v) Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2025 Jul 28 RgGuinier 3.2 nm Dmax 10.9 nm VolumePorod 111 nm3

SASDXG9 – poly(ADP-ribose)polymerase 1 (Af-PARP1) 5'P nicked DNA complex Sample: Aspergillus fumigatus poly(ADP-ribose)polymerase 1 monomer, 74 kDa Aspergillus fumigatus protein 5'Phosphorylated nicked DNA damage mimetic monomer, 14 kDa DNA Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, 2.5% (v/v) Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Nov 14 RgGuinier 4.3 nm Dmax 16.6 nm VolumePorod 190 nm3

SASDXH9 – poly(ADP-ribose)polymerase 1 (Af-PARP1) with N-terminal dBRCT domain truncation:5'P nicked DNA complex Sample: Aspergillus fumigatus poly(ADP-ribosyl)polymerase 1 dBRCT monomer, 58 kDa Aspergillus fumigatus protein 5'Phosphorylated nicked DNA damage mimetic monomer, 14 kDa DNA Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TCEP, 2.5% (v/v) Glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Nov 14 RgGuinier 3.5 nm Dmax 11.4 nm VolumePorod 151 nm3