Human βB2-Crystallin Forms a Face-en-Face Dimer in Solution: An Integrated NMR and SAXS Study.

Xi Z, Whitley MJ, Gronenborn AM
Structure 25(3):496-505 (2017 Mar 7)
PMID: 28238532
doi: 10.1016/j.str.2017.02.001 		Submitted to SASBDB: 2017 Jan 17
Published in SASBDB:

SASDBT9 – Full-length human βB2-crystallin

Beta-crystallin B2 experimental SAS data
DAMMIN model
Sample: Beta-crystallin B2 dimer, 46 kDa Homo sapiens protein
Buffer: 25 mM NaPi, 5 mM DTT, 1 mM EDTA,, pH: 6.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Mar 20
RgGuinier 2.2 nm
Dmax 8.1 nm
VolumePorod 67 nm3

SASDBU9 – Terminally truncated human βB2-crystallin

Beta-crystallin B2 experimental SAS data
DAMMIN model
Sample: Beta-crystallin B2 dimer, 42 kDa Homo sapiens protein
Buffer: 25 mM NaPi, 5 mM DTT, 1 mM EDTA,, pH: 6.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Mar 20
RgGuinier 2.1 nm
Dmax 6.7 nm
VolumePorod 62 nm3