Molecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1

von Velsen J, Juyoux P, Piasentin N, Fisher H, Lapouge K, Vadas O, Gervasio F, Bowler M
(2026 Aug 07)

doi: 10.7554/eLife.111925.1

Submitted to SASBDB: 2026 Jan 18 Published in SASBDB: 2026 Aug 10

SASDY56 – MAPK/ERK Kinase (MEK1) DD - Extracellular signal-Regulated Kinase (ERK2) T185V Sample: MAPK/ERK Kinase (MEK1) DD - Extracellular signal-Regulated Kinase (ERK2) T185V monomer, 86 kDa protein Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2025 Jun 25 RgGuinier 3.1 nm Dmax 11.5 nm VolumePorod 117 nm3

SASDY66 – MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V Sample: MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V monomer, 87 kDa protein Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2025 Jun 25 RgGuinier 3.1 nm Dmax 11.7 nm VolumePorod 134 nm3

SASDY76 – MAPK/ERK Kinase (MEK1) DD - Extracellular signal-Regulated Kinase (ERK2) T185V with ADP.AlF4 Sample: MAPK/ERK Kinase (MEK1) DD - Extracellular signal-Regulated Kinase (ERK2) T185V monomer, 86 kDa protein Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2025 Jun 25 RgGuinier 2.9 nm Dmax 11.0 nm VolumePorod 93 nm3

SASDY86 – MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V with ADP.AlF4 Sample: MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V monomer, 87 kDa protein Buffer: 50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2025 Jun 25 RgGuinier 3.0 nm Dmax 9.6 nm VolumePorod 125 nm3