Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.

Pardo Larrabeiti I, Eibinger M, Esque J, Pradeau S, Fort S, Moraïs S, Mizrahi I, Bayer EA, Nidetzky B, Montanier CY, Roblin P, Dumon C
Protein Sci 35(7):e70649 (2026 Jul)

PMID: 42298785 doi: 10.1002/pro.70649

Submitted to SASBDB: 2026 Feb 27 Published in SASBDB: 2026 Jun 18

SASDYR7 – Glycoside Hydrolase family 9 (AtCel9R) from Acetovibrio thermocellus Sample: ENDOGLUCANASE Cel9R monomer, 71 kDa Acetovibrio thermocellus protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 3.1 nm Dmax 10.2 nm VolumePorod 86 nm3

SASDYS7 – Glycoside Hydrolase family 8 (AtCel8A) from Acetovibrio thermocellus Sample: ENDOGLUCANASE Cel8A monomer, 42 kDa Acetovibrio thermocellus protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 2.0 nm Dmax 6.4 nm VolumePorod 41 nm3

SASDYT7 – Glycoside Hydrolase family 11 (AtXyn11A) from Acetovibrio thermocellus Sample: Endoxylanase Xyn11A monomer, 40 kDa Acetovibrio thermocellus protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 2.9 nm Dmax 10.0 nm VolumePorod 50 nm3

SASDYU7 – Chimeric constructs - CC_1 (AtCel8A_Jo:In-AtCel9R) Sample: Chimeric constructs - CC_1 (AtCel8A_Jo:In-AtCel9R) monomer, 136 kDa Acetovibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 4.6 nm Dmax 17.5 nm VolumePorod 166 nm3

SASDYV7 – Chimeric constructs - CC_2 (Jo_AtCel8A:In-AtCel9R) Sample: Jo_AtCel8A:In-AtCel9R monomer, 136 kDa Acetovibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 4.3 nm Dmax 13.4 nm VolumePorod 172 nm3

SASDYW7 – Chimeric constructs - XCC_A (AtCel8A-Jo-AtXyn11A:In-AtCel9R) Sample: Chimeric constructs - XCC_A (AtCel8A-Jo-AtXyn11A:In-AtCel9R) monomer, 175 kDa Acetovibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.3 nm Dmax 18.7 nm VolumePorod 195 nm3

SASDYX7 – Chimeric constructs - XCC_B (AtCel8A-Jo-AtXyn11A:AtCel9R-In) Sample: Chimeric constructs - XCC_B (AtCel8A-Jo-AtXyn11A:AtCel9R-In) monomer, 175 kDa Acetovibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.4 nm Dmax 20.4 nm VolumePorod 193 nm3

SASDYY7 – Chimeric constructs - XCC_E (AtCel9R-Jo-AtCel8A:AtXyn11A_In) Sample: Chimeric constructs - XCC_E (AtCel9R-Jo-AtCel8A:AtXyn11A_In) monomer, 175 kDa Acetovibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.4 nm Dmax 19.0 nm VolumePorod 240 nm3

SASDYZ7 – Chimeric constructs - XCC_F (AtCel9R-Jo-AtCel8A:In-AtXyn11A) Sample: Chimeric constructs - XCC monomer, 175 kDa protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.4 nm Dmax 18.4 nm

SASDY28 – Chimeric constructs - XCC_K (AtXyn11A-Jo-AtCel9R:AtCel8A-In) Sample: Chimeric constructs - XCC_K (AtXyn11A-Jo-AtCel9R:AtCel8A-In) monomer, 175 kDa Acetivibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.5 nm Dmax 18.0 nm VolumePorod 231 nm3

SASDY38 – Chimeric constructs - XCC_L (AtXyn11A-Jo-AtCel9R:In-AtCel8A) Sample: Chimeric constructs - XCC_L (AtXyn11A-Jo-AtCel9R:In-AtCel8A) monomer, 175 kDa Acetivibrio thermocellus / … protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at XEUSS 2.0, Laboratoire de Génie Chimique on 2025 Apr 4 RgGuinier 5.4 nm Dmax 19.3 nm VolumePorod 245 nm3