Interpreting wide-angle X-ray scattering data using DNA duplexes as model systems.

Hu Q, Amirbekian V, Uttormark S, Higinbotham HR, Pollack L
IUCrJ 13(Pt 5):650-658 (2026 Sep 1)
PMID: 42677922
doi: 10.1107/S2052252526007700 		Submitted to SASBDB: 2026 Mar 2
Published in SASBDB:

SASDYJ9 – SWAXS from AT DNA duplex (25 base pair)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 10 mM MOPS, 150 mM NaCl, 50 µM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Jul 16
RgGuinier 2.2 nm
Dmax 8.5 nm

SASDYK9 – SWAXS from Mix DNA duplex (25 base pair)

Mix-sequence 25 base-paired DNA experimental SAS data
Mix-sequence 25 base-paired DNA Kratky plot
Sample: Mix-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 10 mM MOPS, 100 mM KCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2025 Jun 24
RgGuinier 2.3 nm
Dmax 8.7 nm