Freezing the P-Rex2 DH/PH tandem

Lauren Anderson Submitted to SASBDB: 2026 Apr 3
Published in SASBDB:

SASDZS5 – Frozen human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem (P-Rex2 DH/PH ) with sucrose as cryoprotectant

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem experimental SAS data
BILBOMD model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2026 Feb 17
RgGuinier 2.6 nm
Dmax 8.4 nm
VolumePorod 48 nm3

SASDZT5 – Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem experimental SAS data
BILBOMD model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2026 Feb 17
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 55 nm3