SEC-SAXS characterization of the PPARγ2 AF-1 domain under varying pH and salt conditions

Mithun Karadi

Submitted to SASBDB: 2026 Sep 24 Published in SASBDB: 2026 Oct 7

SASD2H8 – Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain (AF1) in 10 mM sodium phosphate, pH 7.4, containing 500 mM NaCl Sample: Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain monomer, 15 kDa Homo sapiens protein Buffer: 10 mM sodium phosphate, 500 mM NaCl, pH: 7.4 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2025 Mar 17 RgGuinier 3.2 nm Dmax 10.5 nm VolumePorod 23 nm3

SASD2J8 – Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain (AF1) in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl Sample: Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain monomer, 15 kDa Homo sapiens protein Buffer: 10 mM sodium phosphate, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2025 Mar 17 RgGuinier 3.4 nm Dmax 11.7 nm VolumePorod 25 nm3

SASD2K8 – Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain (AF1) in 10 mM sodium phosphate, pH 7.4, containing 50 mM NaCl Sample: Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain monomer, 15 kDa Homo sapiens protein Buffer: 10 mM sodium phosphate, 50mM NaCl, pH: 7.4 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2025 Mar 17 RgGuinier 3.6 nm Dmax 10.2 nm VolumePorod 26 nm3

SASD2L8 – Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain (AF1) in 10 mM sodium phosphate, pH 6, containing 150 mM NaCl Sample: Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain monomer, 15 kDa Homo sapiens protein Buffer: 10 mM sodium phosphate, 150 mM NaCl, pH: 6 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2025 Mar 17 RgGuinier 3.3 nm Dmax 12.1 nm VolumePorod 27 nm3

SASD2M8 – Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain (AF1) in 10 mM sodium phosphate, pH 8, containing 150 mM NaCl Sample: Peroxisome Proliferator-Activated Receptor Gamma Activation Function-1 Domain monomer, 15 kDa Homo sapiens protein Buffer: 10 mM sodium phosphate, 150 mM NaCl, pH: 8 Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2025 Mar 17 RgGuinier 2.8 nm Dmax 10.9 nm VolumePorod 17 nm3